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首页> 外文OA文献 >Visualisation of doxorubicin in human and animal tissues and in cell cultures by immunogold-silver staining.
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Visualisation of doxorubicin in human and animal tissues and in cell cultures by immunogold-silver staining.

机译：免疫金银染色法可在人和动物组织以及细胞培养物中观察阿霉素。

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摘要

In previous pharmacologic studies, the native fluorescent properties of doxorubicin (DOX) have been utilised to visualise tissue and cellular drug distribution. Such distribution studies provide valuable additional information to that obtained by measuring tissue drug concentration alone. An alternative immunocytochemical method of drug localisation using a rabbit immunoadsorbed antiserum to DOX and silver-enhanced gold-labelled second antibodies has been used to achieve visualisation of DOX in normal and malignant tissues from drug-treated animals and patients, and in human tumour cell lines treated in vitro. Non-specific staining in untreated tissues or in controls stained without primary antibody was minimal. Widespread dark brown to black specific immunostaining was observed in the normal tissues of drug-treated animals and in rat sarcoma and in the mouse EMT6 mammary tumour. In human breast tumour biopsy samples obtained at surgery 1 h following a 25 mg intravenous dose of DOX, considerable variation in drug distribution was observed which appeared to be related to drug concentration. Both nuclear and membrane staining was apparent; the latter was especially noticeable in human tumour cells grown in the presence of DOX at concentrations greater than 0.92 microM. Immunolocalisation using silver enhanced gold-labelled reagents provides an additional technique to study cell and organ specific differences in drug uptake and distribution.
机译：在先前的药理研究中，阿霉素（DOX）的天然荧光特性已用于可视化组织和细胞药物分布。这种分布研究为仅通过测量组织药物浓度获得的信息提供了有价值的附加信息。使用兔免疫吸附抗DOX的抗血清和银增强的金标记的第二抗体进行药物定位的另一种免疫细胞化学方法已用于实现药物治疗的动物和患者的正常和恶性组织以及人肿瘤细胞系中DOX的可视化体外处理。未经处理的组织或未经一抗染色的对照中的非特异性染色极少。在药物治疗动物的正常组织，大鼠肉瘤和小鼠EMT6乳腺肿瘤中观察到广泛的深棕色至黑色的特异性免疫染色。在25 mg静脉内剂量的DOX 1小时手术后获得的人乳腺肿瘤活检样品中，观察到药物分布的显着变化，这似乎与药物浓度有关。核和膜染色均很明显。后者在浓度超过0.92 microM的DOX存在下生长的人类肿瘤细胞中尤为明显。使用银增强的金标记试剂进行的免疫定位为研究细胞和器官在药物吸收和分布方面的特异性差异提供了另一种技术。

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    Henneberry, H. P.; Aherne, G. W.;

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  • 年度 1992
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  • 正文语种 {"code":"en","name":"English","id":9}
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